anti slc8a1 antibodies  (Proteintech)

Journal: Toxins

Article Title: Veratridine Induces Vasorelaxation in Mouse Cecocolic Mesenteric Arteries

doi: 10.3390/toxins16120533

Figure Lengend Snippet: Effects of NCX antagonists on VTD-induced vasorelaxation in CAs. Scatter plots illustrating reduction in VTD-induced vasorelaxant responses of CAs with KB-R7943 (10 µM, NCX blocker) ( a ) and with SEA0400 (1 and 10 µM, NCX1 blocker) ( b ). CAs were pre-contracted with U46619 in presence of PZ (1 µM). CAs were isolated from males ( n = 7) and females ( n = 7) ( b ). Values are means ± SEM. Significance was analyzed with paired t test ( a ) and one-way ANOVA, followed by Tukey multiple comparison test ( b ) (****: p < 0.0001).

Article Snippet: The non-specific binding sites were blocked with Tris-buffered saline solution containing 0.1% Tween and 5% BSA for 90 min. Next, the membranes were incubated with rabbit anti-NCX1 (1:500, ANX-011, Alomone Labs, Jerusalem, Israel) or rabbit anti-Pan Na V (1:200, ASC-003, Alomone Labs) primary antibodies overnight at 4 °C.

Techniques: Isolation, Comparison

Journal: Toxins

Article Title: Veratridine Induces Vasorelaxation in Mouse Cecocolic Mesenteric Arteries

doi: 10.3390/toxins16120533

Figure Lengend Snippet: NCX expression in CAs. ( a ) Histograms showing the mRNA expression levels of NCX determined by absolute RT-qPCR in CAs collected from male and female mice. ( b ) NCX1 immunoblotting in the CAs. NCX1 expression was evaluated in CAs by Western blotting with an anti-NCX1 antibody. HSC70 was used as a loading control and mouse brains and hearts were used as positive control samples. The experiments were carried out with 10 µg of proteins. Due to the limited size of the CAs, segments of three mice were pooled. ( c ) NCX1 immunolocalization in CAs. NCX1 were immunodetected in CAs by an anti-NCX1 antibody (red). Endothelium was immunolabeled by an anti-PECAM1 antibody (green). The nucleus was labeled by DAPI (blue). Values are the means ± SEM of three independent experiments. MW: molecular weight.

Article Snippet: The non-specific binding sites were blocked with Tris-buffered saline solution containing 0.1% Tween and 5% BSA for 90 min. Next, the membranes were incubated with rabbit anti-NCX1 (1:500, ANX-011, Alomone Labs, Jerusalem, Israel) or rabbit anti-Pan Na V (1:200, ASC-003, Alomone Labs) primary antibodies overnight at 4 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Positive Control, Immunolabeling, Labeling, Molecular Weight

Journal: Toxins

Article Title: Veratridine Induces Vasorelaxation in Mouse Cecocolic Mesenteric Arteries

doi: 10.3390/toxins16120533

Figure Lengend Snippet: The effects of NCX antagonists on the ACh-induced Ca 2+ response in MS1 ECs. ( a ) RT-qPCR data ( left panel ) illustrated as the RNA relative level (2 −ΔCT ), showing the expression of slc8a1 , encoding NCX1. The undetectable genes ( Slc8a2 and Slc8a3 ) are not illustrated. The Western blot ( right panel ) shows the immunodetection of NCX1 in the MS1 ECs. HSC70 antibodies were used as loading controls. Protein extracts from mouse hearts served as positive controls. ( b ) An example of kinetic traces of the Fura-2 fluorescence–emission ratio, illustrating the effects of ACh (1 µM) co-injected or not co-injected with AP (100 nM), in free Na + and Ca 2+ buffers. The histograms illustrate the normalized emission ratio of Fura-2 measured after Ach injection (1 µM) in Ca 2+ -free buffer and in Na + -free buffer. These data represent the area under the curve (AUC) calculated from the kinetic traces and after normalization by ACh-induced responses at 1 µM in Hank’s Balanced Salt Solution (HBSS), used as the control. ( c , d ) The effects of NCX antagonists on the ACh-induced Ca 2+ response in MS1 ECs. The left panels show examples of kinetic traces of the Fura-2 fluorescence–emission ratio before and after the injection, at 30 s, of ACh, which led to the highest inhibition induced by the two NCX antagonists KB-R7943 (10 µM) ( c ) and SEA0400 (10 µM) ( d ). The graphs on the right panels illustrate the inhibition (% of control) induced by KB-R7943 ( c ) and SEA0400 ( d ), as a function of the ACh concentration. HBSS was used as a negative control ( d ). Data are the mean ± SEM of three independent experiments ( n = 3). Statistical significances were determined using the Mann–Whitney test ( b ) and Wilcoxon test ( c , d ) (* p < 0.05; ** p < 0.01; **** p < 0.0001; ns: non-significant).

Article Snippet: The non-specific binding sites were blocked with Tris-buffered saline solution containing 0.1% Tween and 5% BSA for 90 min. Next, the membranes were incubated with rabbit anti-NCX1 (1:500, ANX-011, Alomone Labs, Jerusalem, Israel) or rabbit anti-Pan Na V (1:200, ASC-003, Alomone Labs) primary antibodies overnight at 4 °C.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunodetection, Fluorescence, Injection, Control, Inhibition, Concentration Assay, Negative Control, MANN-WHITNEY

Journal: Toxins

Article Title: Veratridine Induces Vasorelaxation in Mouse Cecocolic Mesenteric Arteries

doi: 10.3390/toxins16120533

Figure Lengend Snippet: Sequence of primers used for real-time RT-PCR.

Article Snippet: The non-specific binding sites were blocked with Tris-buffered saline solution containing 0.1% Tween and 5% BSA for 90 min. Next, the membranes were incubated with rabbit anti-NCX1 (1:500, ANX-011, Alomone Labs, Jerusalem, Israel) or rabbit anti-Pan Na V (1:200, ASC-003, Alomone Labs) primary antibodies overnight at 4 °C.

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies against SLC8A1 and GAPDH, both at a 1:2000 dilution (Proteintech, Wuhan, China; catalog numbers 12359-1-AP and 60004-1-Ig, respectively).

Techniques:

Journal: Biological Research

Article Title: Inhibition of forward and reverse transport of Ca 2+ via Na + /Ca 2+ exchangers (NCX) prevents sperm capacitation

doi: 10.1186/s40659-024-00535-9

Figure Lengend Snippet: Identification of NCX isoforms. Identification of NCX1, NCX2 and NCX3 in porcine sperm. Representative immunoblots using sperm samples from different boars with A ) anti-NCX1, ( B ) anti-NCX2 and ( C ) anti-NCX3 antibodies, and their respective blocking peptides

Article Snippet: ; Basel, Switzerland) at room temperature and agitation for 1 h. Membranes were subsequently incubated with specific primary antibodies against NCX1 (SLC8A1), NCX2 (SLC8A2), or NCX3 (SLC8A3) (Alomone Labs, Jerusalem, Israel), which were previously diluted in blocking solution at 1:2,000 (v: v), at 4 °C overnight under agitation.

Techniques: Western Blot, Blocking Assay

Journal: Biological Research

Article Title: Inhibition of forward and reverse transport of Ca 2+ via Na + /Ca 2+ exchangers (NCX) prevents sperm capacitation

doi: 10.1186/s40659-024-00535-9

Figure Lengend Snippet: Immunolocalization of NCX1 isoform. Localization of NCX1 in the plasma membrane of porcine sperm ( A – C ), and after the peptide competition assay ( D – F ). NCX1 appears stained in green (Alexa Fluor 488) and nuclei in blue (DAPI; 4′6′-diamidion-2-phenylindole). Scale bar: 15 μm

Article Snippet: ; Basel, Switzerland) at room temperature and agitation for 1 h. Membranes were subsequently incubated with specific primary antibodies against NCX1 (SLC8A1), NCX2 (SLC8A2), or NCX3 (SLC8A3) (Alomone Labs, Jerusalem, Israel), which were previously diluted in blocking solution at 1:2,000 (v: v), at 4 °C overnight under agitation.

Techniques: Membrane, Competitive Binding Assay, Staining

Journal: Biological Research

Article Title: Inhibition of forward and reverse transport of Ca 2+ via Na + /Ca 2+ exchangers (NCX) prevents sperm capacitation

doi: 10.1186/s40659-024-00535-9

Figure Lengend Snippet: Identification of NCX isoforms. Identification of NCX1, NCX2 and NCX3 in porcine sperm. Representative immunoblots using sperm samples from different boars with A ) anti-NCX1, ( B ) anti-NCX2 and ( C ) anti-NCX3 antibodies, and their respective blocking peptides

Article Snippet: To block nonspecific binding sites, samples were incubated with a blocking solution consisting of 5% BSA in PBS at room temperature for 1 h. Subsequently, sperm were incubated with primary NCX antibodies, either NCX1, NCX2 or NCX3 (Alomone Labs), diluted 1:100 (v: v) at room temperature for 1 h. After washing five times with PBS (5 min per wash), samples were incubated with a secondary anti-rabbit antibody Alexa Fluor™ Plus 488 (ref. A32731, Invitrogen, Waltham, MA, USA) diluted 1:200 (v: v) in blocking solution at room temperature for 1 h. Samples were again washed five times with PBS (5 min per wash), air dried and mounted with 10 µL of ProLongTM Glass Antifade Mountant with NucBlue™ (Hoechst 33342; ref. P36985, Invitrogen) in the dark.

Techniques: Western Blot, Blocking Assay

Journal: Biological Research

Article Title: Inhibition of forward and reverse transport of Ca 2+ via Na + /Ca 2+ exchangers (NCX) prevents sperm capacitation

doi: 10.1186/s40659-024-00535-9

Figure Lengend Snippet: Immunolocalization of NCX1 isoform. Localization of NCX1 in the plasma membrane of porcine sperm ( A – C ), and after the peptide competition assay ( D – F ). NCX1 appears stained in green (Alexa Fluor 488) and nuclei in blue (DAPI; 4′6′-diamidion-2-phenylindole). Scale bar: 15 μm

Article Snippet: To block nonspecific binding sites, samples were incubated with a blocking solution consisting of 5% BSA in PBS at room temperature for 1 h. Subsequently, sperm were incubated with primary NCX antibodies, either NCX1, NCX2 or NCX3 (Alomone Labs), diluted 1:100 (v: v) at room temperature for 1 h. After washing five times with PBS (5 min per wash), samples were incubated with a secondary anti-rabbit antibody Alexa Fluor™ Plus 488 (ref. A32731, Invitrogen, Waltham, MA, USA) diluted 1:200 (v: v) in blocking solution at room temperature for 1 h. Samples were again washed five times with PBS (5 min per wash), air dried and mounted with 10 µL of ProLongTM Glass Antifade Mountant with NucBlue™ (Hoechst 33342; ref. P36985, Invitrogen) in the dark.

Techniques: Membrane, Competitive Binding Assay, Staining